Extended Data Fig. 6: CD8 T cells mediate protection of Tap⎯/⎯ chimeric mice against lethal challenge with influenza A virus.
From: TAP dysfunction in dendritic cells enables noncanonical cross-presentation for T cell priming
a, Confocal micrographs at 20X magnification of lung sections from indicated anti-CD8α-treated mice stained for CD8α, CD11c, EpCAM and influenza A virus. Legend to the right shows the color code for each antibody. Scale bar represents 50 µm. b, Lung PR8 viral titers at days 3 and 5 in WT→WT and Tap⎯/⎯→WT chimeric mice. Each symbol is one mouse. c, Pathology scores of infected mice at different time points post lethal PR8 challenge in WT→WT and Tap⎯/⎯→WT mice. Slides were scored by a blinded pathologist for perivascular, bronchiolar or alveolar inflammation, epithelial degeneration or necrosis, and intraluminal debris or hemorrhage. Each symbol is one mouse. d, Hematoxylin-and-eosin staining of lung sections from WT→WT and Tap⎯/⎯→WT mice on days 3 or 5 post PR8 challenge. Scale bar represents 100 μm. The mean ± SEM are presented and each symbol represents a mouse. Data represent 2 experiments (for a total n of 140 mice).
