Extended Data Fig. 2: MDA5 is required for MHV-induced type I interferon expression.
From: TLR2 senses the SARS-CoV-2 envelope protein to produce inflammatory cytokines
(a and b) Real-time PCR analysis of the expression of Ifna (a) and Ifnb (b) in WT, Mda5–/–, and Mavs–/– bone marrow-derived macrophages (BMDMs) after infection with MHV at an MOI of 0.1 for the indicated time, presented relative to levels of the host gene Gapdh. Significant differences compared to the WT group infected with MHV are denoted as ****P < 0.0001 (two-way ANOVA) (a and b). Data are representative of three independent experiments. Data are shown as mean ± SEM (n = 3) (a–b).
