Extended Data Fig. 4: The ferroptosis inhibitor ameliorates lupus progression with much better therapeutic effect compared to the NETosis inhibitor.
From: Glutathione peroxidase 4–regulated neutrophil ferroptosis induces systemic autoimmunity
a-i. MRL/lpr mice (n = 6) were treated with DMSO (0.1 ml 10%), LPX-1(10 mg/kg) or CTX (20 mg/kg) every other day at week 12 for 6 weeks, DMSO (0.1 ml 10%) was applied to sex-matched MRL/Mpj mice (n = 5) as control. Mice were euthanized at 18 weeks of age for analysis. (a) Flow cytometry quantification of lipid ROS. (b) Representative immunofluorescent images of glomeruli stained with IgG (red), IgM (yellow), C1q (green), and DAPI (blue). (c-e) Flow cytometry quantification of plasma inflammatory factors and (f-i) plasma IgG. j-o. MRL/lpr mice (DMSO, LPX-1: n = 3; Cl, Cl+LPX-1: n = 4) were treated with DMSO, Cl, LPX-1, or Cl combined with LPX-1 every other day for 3 weeks starting at the age of week 12. Mice were euthanized at 15 weeks of age for analysis. (j-k) Representative images and related quantification of axillary spleens and lymph nodes. (l) Western blot analysis of cit-H3 in circulating neutrophils from mice subjected to the indicated treatment. (m) Dot plots show the ELISA assessment of serum complement 3. (n) Dot plots show the ELISA assessment of serum anti-dsDNA antibodies titers. (o) Dot plots shows the Bicinchoninic acid (BCA) assay of urine proteins. The scale bar represents 50 μm. Data are shown as mean ± SD. ns p > 0.05. Two-tailed unpaired Student’s t-test was applied.
