Extended Data Fig. 8: Mice with Gpx4 haploinsufficiency in neutrophils developed spontaneous lupus-like disease, while Gpx4 fl/flLysMCre+ mice exhibited mild autoimmunity.
From: Glutathione peroxidase 4–regulated neutrophil ferroptosis induces systemic autoimmunity
a-b. Flow cytometry quantification and western blot analysis of GPX4 in neutrophils (CD45+CD11b+Ly6G&Ly6C+) and non-neutrophils (including monocytes and lymphocytes) from Gpx4fl/fl (n = 6) and Gpx4fl/wtLysMCre+ (n = 9) mice. c-d. Flow cytometry analysis of peripheral neutrophils (CD45+CD11b+Ly6G&Ly6C+) and monocytes (CD45+CD11b+Ly6G&Ly6C-) from Gpx4fl/fl (c: n = 6, d: n = 10) and Gpx4fl/wtLysMCre+ (c: n = 9, d: n = 13) mice. e. Flow cytometry quantification of lipid-ROS and cell viability in neutrophils (n = 6) from Gpx4fl/wtLysMCre+ mice cultured in complete RPMI 1640 basic medium in the presence or absence of LPX-1 (1 μM). f. Skin lesions of Gpx4fl/wtLysMCre+ mice. g. Immunofluorescent images of glomeruli in Gpx4fl/fl mice and Gpx4fl/wtLysMCre+ mice. IgG (red), IgM (yellow), C1q (green), and DAPI (blue). h. Dot plots show the proteinuria of Gpx4fl/fl and Gpx4 fl/flLysMCre+ mice at 4 months of age assessed by BCA assay. i. ELISA assay shows the levels of serum complement 3 in Gpx4fl/fl (n = 8) and Gpx4 fl/flLysMCre+ (n = 8) mice at 6 months of age. j. ELISA assay shows the levels of serum anti-dsDNA antibodies in Gpx4fl/fl (n = 8) and Gpx4 fl/flLysMCre+ (n = 8) mice at 6 months of age. The scale bar represents 50 μm. Data are shown as mean ± SD, ns p > 0.05. Two-tailed unpaired or paired Student’s t-test was applied.
