Extended Data Fig. 4: High cytosolic ROS expression in TFH cells.
From: Selenium–GPX4 axis protects follicular helper T cells from ferroptosis
a, Flow cytometric analysis of cytosolic ROS production in purified CD44+CD25–CXCR5highPD-1high TFH and CD44+CD25–CXCR5–PD-1– non-TFH cells from spleen of SRBC-immunised mice at day 8 post immunisation using CM-H2DCFDA (n = 3). Each dot represents one mouse. b, Comparison of cytosolic ROS levels between circulating CD25–/lowCD127–/+CD45RA–CXCR5+ TFH and CD25–/lowCD127–/+CD45RA–CXCR5– non-TFH effector cells in human peripheral blood (n = 5). Each dot represents a single blood donor. c, Comparison of cytosolic ROS levels between CD45RA–CXCR5high TFH and CD45RA–CXCR5– non-TFH effector cells in human tonsils (n = 10). Each dot represents a single tonsil donor. Data are analysed by paired-sample t-test.
