Extended Data Fig. 5: Comparing the levels of ferroptosis-related factors in TFH and non-TFH cells.
From: Selenium–GPX4 axis protects follicular helper T cells from ferroptosis
a, Gene expression related to cytosolic and lipid ROS and ferroptosis in TFH and non-TFH cells in human and mice. Data were obtained by analysing published RNA-seq data. b, Glutathione (GSH) levels in purified tonsillar CD4+CD19–CD25–CD45RA–CXCR5high TFH and CD4+CD19–CD25–CD45RA–CXCR5– non-TFH cells were detected by ELISA (n = 3). c, Total Fe levels in purified tonsillar TFH and non-TFH cells were analysed by ICP-AES (n = 4). d, Fe2+ levels in tonsillar TFH and non-TFH cells were analysed by flow cytometry using FerroOrange (n = 5). e, Total SFAs and the ratio of total PUFAs/total MUFA in purified tonsillar TFH and non-TFH cells were analysed by GC-MS (n = 4). For b-e, each dot represents a single tonsil donor. Data are analysed by paired-sample t-test.
