Extended Data Fig. 2: CD127− cytotoxic-like cells are bona-fide ILC1s.
From: Effector differentiation downstream of lineage commitment in ILC1s is driven by Hobit across tissues
a Dot plot representation of selected ILC1 and NK cell marker gene expression associated with the clusters identified in Fig. 1c. Color indicates z-score of mean expression across clusters and dot size represents fraction of cells in the cluster expressing the respective gene. b, c, f Frequency of liver ILC1s expressing indicated proteins in NKp46Cre versus NKp46Cre Eomesfl/fl mice (b), WT versus Rag1-/- mice (c) and SPF versus germ free mice (f). Data are representative of 2 independent experiments with n=2 and 3 mice per group (b, c, f). d, e Representative FACS analysis of liver ILC1 of RORc-eYFP fate mapper (FM) mice. (d) FACS analysis and bar graph shows frequency of RORc fate map-positive cells within CD127+ and CD127− liver ILC1 and NK cells. Data are representative of 2 independent experiments with n=3 mice per group. (e) FACS plots show marker protein expression of RORc fate mapper-positive (top) and negative (bottom) liver ILC1s. Bar graphs show frequency of Gzmb-positive cells within FM-positive and FM-negative ILC1s. Data are pooled from 2 independent experiments with n=2 mice per group. Bar graphs indicate individual mice (symbols) and mean (bar), error bars display means ± s.d. Statistical significance was calculated by unpaired two-tailed t-test; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
