Extended Data Fig. 5: Hobit drives the development and effector maturation of hepatic ILC1s and is expressed in committed ILC1s.
From: Effector differentiation downstream of lineage commitment in ILC1s is driven by Hobit across tissues
a-c Representative FACS analysis of liver ILC1s in mixed bone marrow chimeric mice containing a congenically marked WT and HobitKO compartment (a). Frequency of liver ILC1 expressing indicated marker (b) and gMFI of CD127, TCF-1 and IL-18R1 expression within the respective marker-positive cells (c). Data are representative of 3 individual experiments with n=4 mice per group (a-c). d-g Analysis of an unbiased ILCP core signature derived from a scRNA-seq dataset of M progenitors generated by Harly et al29. Violin plots display ILCP score calculation and heat maps display individual gene expression across identified clusters (d, f) and in the dataset generated by Harly et al29(e, g). ALP - all-lymphoid progenitors, sEILP - specific early innate lymphoid progenitors, cEILP - committed early innate lymphoid progenitors, ILCP - innate lymphoid progenitors. Bar graphs indicate individual mice (symbols) and mean (bar), error bars display means ± s.d. Statistical significance was calculated by unpaired two-tailed t-test; **P < 0.01, ***P < 0.001, ****P < 0.0001.
