Extended Data Fig. 9: Id2iΔRORγt/Tbet mice are able to control and clear Citrobacter rodentium infection.
From: Reciprocal transcription factor networks govern tissue-resident ILC3 subset function and identity
To functionally test whether protective intestinal mechanisms were maintained in Id2iΔRORγt/Tbet mice, infection with Citrobacter rodentium was used. a C. rodentium counts expressed as CFU/g feces over the time course of infection in Id2iΔRORγt (n = 4) and Id2iΔRORγt/Tbet (n = 5 or 6 depending on the time point) mice versus littermate ‘no cre’ controls (n = 8; to ensure comparable microbiome). b Relative expression of RegIIIb and RegIIIg in Id2iΔRORγt and Id2iΔRORγt/Tbet mice versus littermate ‘no cre’ controls at 21 dpi with C. rodentium (no cre control: n = 8; Id2iΔRORγt: n = 4; Id2iΔRORγt/Tbet: n = 6). c Histological analysis of colon isolated from Id2creERT2, Id2iΔRORγt and Id2iΔRORγt/Tbet mice at 15 dpi with C. rodentium (n = 3 for each strain, one representative picture from one representative animal per strain). Each data point on graphs is a mouse, bars denote the mean with error bars denoting the SEM. Statistical significance was tested using a one-sided Kruskal-Wallis test with a Dunn’s test for multiple comparisons. *P ≤ 0.05. Exact P values are provided in the source data.
