Extended Data Fig. 1: ILC3 subset-specific deletion of TFs reveals that LTi-like cells express normal levels of IL-22 in the absence of both RORγt and RORα.

Il-17a^cre mice were used to enable ILC3 subset specific targeting of TF-deletion. a Gating strategy for identification of tdRFP⁺ ILCs from SILP of Il17a^cre mice. b Expression of RORγt versus NKp46 by tdRFP⁻ and tdRFP⁺ ILCs from SILP of Il17a^cre mice. c Proportion of ILCs in the SILP of Il17a^cre mice that express tdRFP at different ages (wks) post birth. d Representative CCR6, CD4, c-Kit, NK1.1 and NKp46 expression by tdRFP⁺ ILCs from SILP of Il17a^cre mice. e Proportion of tdRFP⁺ ILCs from SILP of Il17a^cre mice expressing molecules assessed in ‘d’. f Representative expression of RORγt versus CD127 by tdRFP⁻ and tdRFP⁺ ILCs from SILP of Il17a^cre, Il17a^ΔRORγt and Il17a^{ΔRORγt/RORα} mice. g Percentage of tdRFP⁻ and tdRFP⁺ ILCs expressing RORγt (Il17a^cre: n = 12; Il17a^ΔRORγt: n = 11; from 4 independent experiments). h Representative expression of RORγt versus IL-17A and IL-22 by tdRFP⁺ ILCs from SILP of Il17a^cre, Il17a^ΔRORγt and Il17a^{ΔRORγt/RORα} mice. i Total number of tdRFP⁺ ILCs from SILP of Il17a^cre, Il17a^ΔRORγt and Il17a^{ΔRORγt/RORα} mice expressing IL-17A (top) and IL-22 (bottom) (Il17a^cre: n = 19; Il17a^ΔRORγt: n = 14; Il17a^{ΔRORγt/RORα}: n = 19; from 6 independent experiments). Each data point on graphs is a mouse, horizontal bars denote the median. Statistical significance in ‘g’ was tested using unpaired two-tailed Student’s t tests, and in ‘i’ an ordinary one-way ANOVA with Tukey’s test for multiple comparisons. ns = not significant, **P ≤ 0.01, ****P ≤ 0.0001. Exact P values are provided in the source data.

Source data