Fig. 7: Quantitative analyses of ISG15-dependent responses in SARS-CoV-2-infected macrophages.
From: Altered ISGylation drives aberrant macrophage-dependent immune responses during SARS-CoV-2 infection
a, iPSC-derived macrophages were either transfected with NT DsiRNA or those targeting ISG15 or UBE1L. Conditioned medium was collected from control, SARS-CoV-2-infected or IFN-γ-treated cells. Proteins were extracted from each of the samples, separated by SDS–PAGE and digested with trypsin for LC–MS/MS as described in the Methods. The total numbers of proteins were quantified in at least two biological replicates. b, PCA was performed using the Perseus software. The filled squares represent control cells (NT DsiRNA), the empty squares represent ISG15-depleted, the filled circles represent UBE1L-depleted. Uninfected cells are shown in gray and infected cells are shown in red. c, The heatmap represents the hierarchical clustering of the common proteins in the secretome for IFN-γ-treated or SARS-CoV-2-infected cells. The color key represents changes (log2 scale) from dark blue indicating the largest decreases to red indicating the largest increases. d, Functional annotation of the common proteins identified in all samples was performed using the DAVID software.
