Extended Data Fig. 3: Morphological mitochondrial changes but not numbers in IECs post allo-HCT.
From: Mitochondrial complex II in intestinal epithelial cells regulates T cell-mediated immunopathology
C57BL/6 animals received HCT (BALB/c or C57BL/6 → C57BL/6). (a) Immunoblot of SDHA, complex V, III, IV, SDHB, I and TOM-20 in mitochondria from colonic IECs 21days post HCT (n = 4). (b) Immunoblot and protein density quantification of SDHA, complex I, III, IV, V, and TOM-20 in mitochondria from colonic IECs 7days post HCT (n = 4). (c) Representative images of immunofluorescence staining of kidney and liver from recipients 21days post HCT (Complex IV = green, SDHA = red, DAPI = blue, scale bar= 50 µm, n = 5). (d) Numbers of gold particles per mitochondrial from colon and ileum of naive animals or recipients 7days post HCT in transmission electron microscopy images with immune-gold staining of SDHA (total 50 mitochondria from 3 samples). (e) Representative images of transmission electron microscopy in mitochondria of colon from naive or recipients 21days post HCT (scale bar =200 nm). Arrow indicates normal cristae and arrowhead indicates abnormal cristae (n = 3). (f) Mitochondria DNA relative copy numbers of colon and ileum from syngeneic and allogeneic recipients 7 and 21days post HCT (n = 5). All data are from biologically independent animals. Representative plots and a graph summarizing the results of at least two independent experiments are shown. All statistical analysis by two-tailed Mann-Whitney test (b) or one-way ANOVA analysis with Tukey post hoc test (d) (mean ± s.e.m.).
