Extended Data Fig. 2: Genetics and cross-reactivity of characterized WRAIR NTD and RBD mAbs.
a, Gene assignment was performed with IgBlast using Kabat numbering. SHM, somatic hypermutation; CDR, complementarity determining region; VDJ, variable, diversity and joining genes. IC50 values from the pseudovirus neutralization assay, as well as competition groups are indicated. Only two clonally related mAbs were identified, WRAIR-2008 and -2037. b, c, Binding of WRAIR NTD (b) and RBD (c) mAbs to a panel of 26 human CoV and HIV control (gp140 and gp41) antigens, assessed in a multiplex bead-based assay. Binding magnitude is expressed as signal/noise (S/N) ratio, with noise calculated from an HIV mAb antibody control (MZ4). S/N ≥ 10 (dotted line) were considered positive based on negative control binding. d, SARS-CoV-1 (Sino1-11) neutralization activity in a pseudotyped assay. Data are mean from two independent experiments. WRAIR-2063 is indicated in green with the IC50 (µg ml-1) indicated in parenthesis. The CR3022 positive control is indicated in black. Other WRAIR RBD and NTD mAbs that did not neutralize SARS-CoV-1 are indicated in grey.
