Extended Data Fig. 2: TCR clonotypes for single cells and T cell clones and EC50 derivation. | Nature Immunology

Extended Data Fig. 2: TCR clonotypes for single cells and T cell clones and EC50 derivation.

From: An immunodominant NP105–113-B*07:02 cytotoxic T cell response controls viral replication and is associated with less severe COVID-19 disease

Extended Data Fig. 2

(a) Flow diagrams to show T cell clonotypes (defined as TRBV gene usage and CDR3β sequence) between SmartSeq2 sequenced ex vivo single cells (ex vivo single T cells column) and bulk TCR sequenced T cell clones grown in vitro culture (T cell clones column). Panel on the left shows grouped TCRs from patients with mild disease, panel on the right for patients with severe disease. (b) Upper panel: IFN-γ ELISPOT assay for representative high and low functional avidity clones for each patient in blue and red respectively (C-COV19-038 only has low functional avidity clone shown). Lower panels: high functional avidity clone from C-COV19-45 and low functional avidity clone from C-COV-038 with example of EC50 derivation.

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