Extended Data Fig. 5: Loss of iRHOM2 in colon epithelial cells is compensated for by iRHOM1 expression.
(a) Scatter plot of principal coordinates analysis axis one (PC1) and axis two (PC2) performed on North American healthy control and patients’ microbiome profiles. Individual data points are colored according to patients and HC. (b) Composition of microbes in patient stool organized by identified families of bacteria (c) Crypt length in colons of mice with and without infection by C. rodentium (n = 3 for each WT and KO groups). (d) The number of goblet cells in 500 µm2 in colons of mice (n = 3 for each WT and KO groups). (e) Q-PCR of RHBDF1 and RHBDF2 in SW620 cells treated with the indicated sgRNAs or control RNA. 18 S was the endogenous control. RQ, relative quantitation (n = 3 per group). All data are mean ± s.d. and were analyzed by two-tailed, unpaired Student’s t-test (c-e).
