Extended Data Fig. 3: ILC3s express HB-EGF in the large intestine.

a, Flow cytometry plots show HB-EGF expression in large intestine lamina propria (LI-LP) of noted mice as measured by conversion of the fluorescent LacZ substrate fluorescein di-β-d-galactopyranoside (FDG). b, Graph of frequency of HB-EGF⁺ cells in SI-LP of noted mice as measured by LacZ activity (n = 5 individual mice). c–e, Flow cytometry plots (c), graph of frequency (d) and quantification of cell number (e) of HB-EGF⁺ cells in LI-LP of noted mice as measured by LacZ activity (n = 4 individual mice). In both small and large intestines, CD4⁺ T cells were gated on CD45⁺CD3ε⁺CD4⁺, macrophages were gated on CD45⁺CD11b⁺CD11c⁺MHC II⁺CD64⁺, dendritic cells were gated on CD45⁺CD11b⁺CD11c⁺MHCII⁺CD64^-, ILC2s were gated on CD45⁺Lin^- CD127⁺CD90.2⁺KLRG1⁺, ILC3s were gated on CD45⁺Lin^-CD127⁺CD90.2⁺KLRG1^-CD45^dim CCR6⁺. Lineage 1: CD3ε, CD5, CD8α, NK1.1, TCRγδ. Lineage 2: CD11b, CD11c, B220. Data in a-e are representative of two or three independent experiments, shown as the means ± S.E.M. Statistics are calculated by one-way ANOVA.

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