Extended Data Fig. 4: HB-EGF protects from TNF-induced intestinal epithelial cell death.
a–c, Cell death was induced in the murine rectum epithelial cell line CMT-93 by TNF and a cIAP inhibitor. Cell death was determined by representative flow cytometric analysis of cleaved-caspase 3 (a) or cleaved-caspase 8 (c) in the presence or absence of recombinant HB-EGF. Cell death was determined by representative flow cytometric analysis of cleaved-caspase 3 in the presence of various inhibitors (b). Data in a and b are representative of three independent experiments, data in c are pooled from three independent experiments, shown as the means ± S.E.M. Statistics are calculated by one-way ANOVA.
