Extended Data Fig. 10: IL-1β is a key factor for antitumor immune responses.
From: Recruitment and activation of type 3 innate lymphoid cells promote antitumor immune responses
a, Flow cytometry analysis of Caspase-1 cleavage in myeloid cells MSC-2 treated in vitro with LPS and CDDP or LPS and Nigericin as a positive control for 24 h. n = 6. b, IL-1β production by myeloid cells MSC-2 treated in vitro with LPS and CDDP or LPS and Nigericin as a positive control for 24 h. n = 4. c-g, In vivo Flow cytometry analysis of Pro-IL-1β expression in immune cells from TC-1 tumors seven day after a CDDP or a PBS treatment Results show the numbers of cells from 300 000 total viable cells from each tumor. n = 5. Numbers of c, eosinophils, d, macrophages, e, M-MDSCs, f, PMN-MDSCs, g, DCs. h, Representative flow cytometry plot of CD69 expression in ILC3s. i, Therapeutic response of WT and TCRδ-/- mice bearing TC-1 tumors treated with CDDP or PBS reflected by tumor growth following the experimental setting depicted above. Flow cytometry analysis of the numbers of j, CD4+ and CD8+ cells of MC38 tumors from WT mice treated with ICB and IL-1RA as in Fig. 6l. n = 5. Numbers are shown for 300 000 total viable cells from each MC38 tumor. Therapeutic response of k, littermate controls Rorcfl/flCd4+/+ mice, n = 5, and l, Rorc–/– mice, n = 5, bearing MC38 tumors to ICB in combination with IL-1RA reflected by tumor growth following the experimental setting depicted above each data. m, Therapeutic response of WT mice bearing a MC38 tumor treated with rIL-1β and rCCL20 alone or in combination with ICB, reflected by tumor growth following the experimental setting depicted above, n = 5. All tumor growth results are shown as mean ± SEM and experiments followed the experimental setting depicted above each data or referred to the main figures and are representative of three independent experiments.; other data are shown as mean ± SD and are representative of three independent experiments. Statistical significance was determined using one-way anova (a, b), two-way anova and Tukey’s multiple comparison test (c–g, i–m).
