Extended Data Fig. 6: In situ hybridization workflow and supplemental analysis.
From: The immunoregulatory landscape of human tuberculosis granulomas
a, Conceptual overview of combined ISH and MIBI-TOF experimental workflow. b, Representative images of control probes UBC ( + ) and DapB (-) in HeLa cell pellets. c, Representative images of all probes in human spleen and melanoma at 40x magnification. d, Grouped bar plots of total counts (left), area-normalized counts (middle), and cell-normalized counts (right) for all granuloma regions analyzed. e, The ratio of TGFB: IFNG transcripts as a log2 fold-change for total counts (left), area-normalized counts (middle), and cell-normalized counts (right) for all granuloma regions analyzed.
