Extended Data Fig. 7: Immunoregulatory protein expression in nontuberculous granulomas.

a, Hematoxylin & eosin-stained sections of sarcoidosis granuloma FOVs. b, Frequency of immune cell subsets out of total immune cells broken down by sarcoidosis FOV. c, Comparison of cell type frequency (out of total cells) between tuberculosis (dark green) and sarcoidosis (light green). Boxplots display the median and interquartile range (IQR, 25-75%) with whiskers representing the upper- and lower-quartile ± 1.5*IQR (TB n = 30, sarcoid n = 10). P-values were determined with a Wilcoxon Rank Sum Test (two-tailed) where: ns p > 0.05, * p < 0.05, and ** p < 0.01. d, Frequency of PD-L1⁺ cells across all sarcoidosis FOVs broken down by cell subset. e, Representative immunohistochemistry images of PD-L1 or IDO1 (brown) of controls (top = spleen, bottom=placenta), a sarcoid granuloma, xanthoma granuloma, foreign body lesion, and endometrial lesion with hematoxylin nuclear counterstaining (purple). f, Hematoxylin and eosin (left) and MIBI-TOF staining for major cell lineage markers (middle) or IDO1 (magenta) and PD-L1 (cyan) (right) of a representative pulmonary Mycobacterium avium FOV.