Extended Data Fig. 3: Impact of LRF on IELp homing.

(a) Graph shows the absolute number of the indicated TCRβ⁺ tester cells (Ctrl or LRF KO) among IEL or spleen T cells analyzed in Fig. 3ab. Data are from one experiment (5 mice per group) representative of two. (b) CD8α vs. CD8β expression on TCRαβ⁺ CD8α⁺ splenocytes from bone marrow chimera analyzed in Fig. 1e. Graph (right) shows the percentage of CD8αα cells among TCRαβ⁺ CD8α⁺ splenocytes. Data pooled from two independent experiments with a total of 6 mice per group. Each symbol represents one mouse. (c-e) NSG host mice were adoptively transferred with a 1:1 mixture of CD45.2⁺ tester (either Ctrl or LRF KO) and CD45.1⁺CD45.2⁺ competitor CD8αβ splenocytes, and analyzed one week after transfer. Data are from one experiment (5 Ctrl and 4 KO mice) representative of two. (c) Schematic of the experiment (d, e) Top contour plots show CD45.2 vs. CD45.1 expression in TCRβ⁺ CD45.2⁺ splenocytes (d) and IEL (e). Colored boxes define Ctrl (blue) and KO (red) testers populations assessed for CD8α vs. CD8β expression in bottom contour plots. Top right graphs show tester/competitor ratios in each organ. Each symbol represents one mouse. Bottom right panel in (e) shows CD8α vs. CD8β expression on CD8α⁺ IEL from an unmanipulated C57BL/6 (WT) mouse. Error bars indicate standard error of the mean (SEM). P values (a, b, d, e) are from two-tailed unpaired t-test.

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