Fig. 4: Single-cell transcriptional response induced by BNT162b2 and YF-17D immunization in dLNs.
From: Mechanisms of innate and adaptive immunity to the Pfizer-BioNTech BNT162b2 vaccine
a, Experimental design and timeline. b, UMAP of cell types clustered by single-cell transcriptional analysis of 52,788 cells after quality control. MDDCs, monocyte-derived dendritic cells. c, DEGs induced by BNT162b2 and YF-17D immunization at days 1, 3 and 7. d, Significantly enriched interferon and cell cycle BTMs (false discovery rate < 0.05, absolute normalized enrichment score (NES) > 2) across all clusters from days 1 to 7 after BNT162b2 immunization. Only clusters with a significantly modulated pathway are shown. e, UMAP of subclusters in C2 (mono_macrophage) and C15 (macrophage) after BNT162b2 and YF-17D immunization, respectively. f, Heat map of key interferon response and AP-1 transcription factors after BNT162b2 immunization. Samples used for scRNA-seq were pooled from three independent experiments containing 8–10 mice.
