Extended Data Fig. 1: Immune response induced by low immunization dose in mice.
From: Mechanisms of innate and adaptive immunity to the Pfizer-BioNTech BNT162b2 vaccine
a, S binding titers induced by low dose (0.2 μg/mouse) of BNT162b2 immunization measured by ELISA. b, Class I tetramer specific CD8 T cell response in lung and spleen of mice measured at days 21 and 42 after BNT162b2 immunization (0.2 μg/mouse). c-d, Antigen specific CD8 (c) and CD4 (d) T cell response in lung and spleen of mice detected at days 21 and 42 by intracellular cytokine staining (ICS) assay. e, Comparison between the activation of innate cells in dLNs induced by low (0.2 μg/mouse) and high immunization doses (5 μg/mouse). f, Cytokines/chemokines produced at 6 h post prime with low (0.2 μg/mouse) dose detected by Luminex assay. Data were combined from two independent experiments (a) or one representative experiment (b-f). One-Way ANOVA followed by Tukey’s test was applied (a-e). P-values were determined using Student’s t-tests (two-tailed) (f). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
