Extended Data Fig. 7: (a) Western blot and (b) normalized (MYC/β-Actin) expression after densitometry analysis (n = 3) showing that MYC protein in Treg was significatively increased at 2 hr and persisted for up to 48 hr post single injection of mIL-2/CD25. | Nature Immunology

Extended Data Fig. 7: (a) Western blot and (b) normalized (MYC/β-Actin) expression after densitometry analysis (n = 3) showing that MYC protein in Treg was significatively increased at 2 hr and persisted for up to 48 hr post single injection of mIL-2/CD25.

From: Dynamic transcriptional activity and chromatin remodeling of regulatory T cells after varied duration of interleukin-2 receptor signaling

Extended Data Fig. 7

Protein extracts were obtained from FACS-sorted CD4 + Foxp3 + Tregs (>95% Foxp3 + ) isolated from C57BL/6J-Foxp3 + RFP mice at the indicated times after PBS, or mIL-2/CD25 (20 µg) injection. Expression levels of MYC and β-actin were analyzed by Western blotting with anti–MYC (10828-1-AP), or anti-β Actin (20536-1-AP) and revealed with a goat anti-rabbit polyclonal (G-21234). (b) One-way ANOVA with Tukey’s multiple comparisons test, ****p < 0.0001; ***p = 0.0002; n.s p = 0.1131. (c) RNA-seq time course expression of Myc or Slc7a5 in response to mIL-2/CD25. Data shown in (a) is from one representative gel where lanes were spliced to move relevant data neighboring to each other.

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