Extended Data Fig. 2: Representative FACS gating strategy for sorted Treg at 72 hr post single injection of mIL-2/CD25.

CD4 + T cells from the spleen of CD4 + Foxp3 + -RFP + reporter mice were enriched with anti-CD4 magnetic beads and stained with FITC - anti-CD4 antibody. RFP + Treg were sorted as shown. Treg purity was typically greater than 98%.