Extended Data Fig. 4: Functionality of neutrophils derived from NCPs, as well as CD15 expression by both neutrophil and monocyte precursors among BM-LDCs and their mature counterparts.
From: CD66b−CD64dimCD115− cells in the human bone marrow represent neutrophil-committed progenitors
a, Histogram plot showing the percentages and the type of colonies generated after 14 days of NCP and BM-LDC culture (n = 3). Data represent means ± s.e.m. b, Representative plot showing the time-course of O2− production by neutrophils derived from NCPs cultured for 7 days with SFGc (orange, green, magenta and turquoise) as compared to blood neutrophils (red) (n = 3). c, Representative cytospins showing the phagocytosis of zymosan particles by neutrophils derived from NCPs cultured for 7 days with SFGc (n = 3). d,e, Histogram overlays showing the expression of CD15 by NCPs (orange, green, magenta and turquoise), PMs (purple), SNs (black), cMoPs (light blue), pre-monocytes (blue) and CD14+ monocytes (red) as compared to the fluorescence-negative control (light gray) (n = 5).
