Extended Data Fig. 7: dTGN colocalization and biochemical pro-caspase-11–NLRP3 interaction.
From: Caspase-11 interaction with NLRP3 potentiates the noncanonical activation of the NLRP3 inflammasome
a,b, Immunofluorescence confocal microscopy 16 h post stimulation of WT macrophages with L or HK virulent E. coli (a), and WT, Casp11–/– and Nlrp3–/– macrophages as indicated with L E coli (b). Scale bar=10 µm. In (a), side micrograph insets in the triple merges show magnification of the indicated areas. c,d, Immunoprecipitation (IP) of endogenous caspase-11 or NLRP3 as indicated, and immunoblotting for co-immunoprecipitating proteins and WCE proteins (labels to left of immunoblot panels) from macrophages stimulated 12 h with L, HK or HK E. coli supplemented with indicated dose of E. coli RNAtot. (c) and L, HK or HK E. coli supplemented with indicated dose of E. coli RNAtot or Nigericin, E. coli RNAtot alone or Nigericin alone (d). Bacteria:macrophage=20:1. Results represent at least three independent experiments.
