Extended Data Fig. 8: GSDMD is important for noncanonical activation of the NLRP3 inflammasome in response to Gram-negative bacteria.
From: Caspase-11 interaction with NLRP3 potentiates the noncanonical activation of the NLRP3 inflammasome
a–c, Immunoblots of macrophage concentrated supernatants (20 h) or WCE at 6 h (a,b) and 20 h (c) post stimulation of WT and Gsdmd–/– macrophages with L or HK E. coli (avirulent) or virulent E. coli as indicated. (d) Immunoblots of macrophage concentrated supernatants (20 h) or WCE (6 h) post stimulation of macrophages with L or HK E. coli with or without zVAD-FMK treatment.
