Extended Data Fig. 1: Bacterial mRNA and stimulatory LPS are both required for caspase-11 and noncanonical inflammasome activation.
From: Caspase-11 interaction with NLRP3 potentiates the noncanonical activation of the NLRP3 inflammasome
a, Immunofluorescence confocal microscopy on bone marrow derived macrophages (hereafter ‘macrophages’) 2 h post stimulation with L or HK E. coli or untreated. b, Bar graph, % cells with LAMP1-LPS colocalization. c, Immunoblots of macrophage concentrated supernatants (20 h) or WCE (6 h), and cytokine concentrations and LDH release in culture supernatants (20 h) post stimulation with L, HK or HK E. coli supplemented with 100 ng ml–1 mRNA isolated from E. coli or eukaryotic cells. LDH measured by cytotoxicity assay; IL-1β, TNF and IL-6 by ELISA. Error bars, mean ± s.e.m. t-test was performed in b (n = 3). ns: nonsignificant. Bacteria:macrophage=20:1. Results represent at least three independent experiments.
