Extended Data Fig. 8: Network analysis reveals differences in possible interactions between Lag3ΔTM and Cre Control.
From: Autoreactive CD8+ T cells are restrained by an exhaustion-like program that is maintained by LAG3
(a-c) CD8+ T cells from the islets and ndLN were isolated from 4 Cre Control and 4 Lag3∆TM 8-week NOD female mice and were subjected to 5’ paired single cell RNAseq (scRNAseq) and single cell T cell receptor sequencing (scTCRseq). Unless otherwise noted, red is representative of Lag3∆TM seed genes (Clusters 3 + 4) and blue is representative of Cre Control seed genes (Cluster 6) (Supplementary Table 4). For subnetworks, all gene names are shown. (a) Protein subnetworks characterizing Cre Control (b) Protein subnetworks characterizing Lag3∆TM cells (c) Degree distribution for the different subnetworks showing that Cre Control have a higher frequency of networks with fewer connections.
