Extended Data Fig. 4: CD49a⁺CD103⁺ ILC1s and CD49a⁻CD103⁻ NK cells are phenotypically distinct in terms of NKG2A expression.

a, Violin plot showing KLRC1 expression in the indicated clusters. b, (Left) Representative histograms of NKG2A expression in CD49a^-CD103⁻ NK cells (green) and CD49a⁺CD103⁺ ILC1s (orange) from the same patient of the indicated histology. (Center) Quantification of NKG2A⁺ cells within the indicated cell type and histology. (Right) MFI of NKG2A in NKG2A⁺CD49a⁺CD103⁺ ILC1s compared to NKG2A⁺CD49a^-CD103⁻NK cells in tumor samples. chRCC n = 6, ccRCC n = 9. Each pair of symbols connected by a line denotes an individual patient. Two-tailed unpaired t test was used for statistical analysis of the percent NKG2A positive, and paired ratio t test was used for statistical analysis of MFI, ***p < 0.001, ****p < 0.0001. c, Violin plot showing log-normalized expression of the HLA-E gene in the TCGA ccRCC and chRCC cohorts. Two-sided Wilcoxon test was used for statistical analysis p < 2.2e^-16. d, Correlation between level of HLA-E expression and ILC1 signature in chRCC cases from the TCGA database. Statistical analyses calculated using Spearman’s correlation. Error bands represent the 95% confidence interval. e, Association of HLA-E expression and overall survival across the TCGA chRCC cohort. High represents the top quartile and low represents the bottom 3 quartiles of IL-15 expression level. P value calculated using a Cox regression and log-rank test.