Extended Data Fig. 1: Cluster-defining marker plots for all clusters and heatmap of differential gene expression analysis among the three CD8+ T cell clusters.

a, Marker plots showing normalized expression of selected common markers for lymphoid and myeloid populations (CD3D – T cells, CD4 – CD4+ T cells, CD8A – CD8+ T cells, KLRB1 – innate lymphocytes, CD14 – myeloid cells). b, Heatmap of expression of the top 30 DEGs by log fold change, [computed using Wilcox test in the FindMarkers() function of Seurat (FDR P < 0.05 and LFC > 0)], across the three CD8+ T cell clusters within the chRCC and ccRCC patients. Each column represents an individual cell. c, Plots showing the back gating strategy for plots shown in Fig. 1e.