Fig. 2: GBMs are composed of transcriptionally distinct populations of EGFR⁺ cancer cells.

a, Area (in mm²) of EGFR⁺ cell clusters at 7, 14 and 21 days post Cre virus injection. Data are presented as mean ± s.e.m. of biologically independent tumors, *P < 0.0001, unpaired t test, two-tailed, n = 9 (three or four sections per tumor), 16 and 11 for 7, 14 and 21 days, respectively. b, Confetti sections of a > 30 days late-stage GBM. Scale bars, left panel 100 μm, right panel 500 μm. NB, normal brain. c, Fluorescent cells (A and B) or clusters (C and D) from biologically independent mice bearing early-stage (10 days) and late-stage (>30 days) post Cre virus injection. Data are presented as mean ± s.e.m. of n = 4–6 fields of view per section and n = 4–5 sections per biologically independent tumors were quantified. d, Monocle3 trajectory inference on EGFR⁺ clusters. e, DEGs between EGFR⁺ clusters 0 and 5. NS, not significant. f, Expression of EGFR ligands Hbegf and Tgfa overlaid on UMAPs. g, Expression of Lgals1 (GAL1) and Pdgfra overlaid on UMAPs. h,i, Heat map (h) and volcano plot (i) of DEGs from bulk RNAseq of hsEGFR⁺PDGFRA⁺ and hsEGFR⁺GAL1⁺ flow-sorted cells. j, Reactome analysis of upregulated genes in hsEGFR⁺PDGFRA⁺ and hsEGFR⁺GAL1⁺ cell populations.

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