Extended Data Fig. 2: A hypoxic environment induces hypoxaemia with equivalent circulating neutrophil.
From: Hypoxia shapes the immune landscape in lung injury and promotes the persistence of inflammation
(a) Schematic of normoxic and hypoxic LPS-induced ALI. (b) Oxygen saturations in mice were measured in mice at baseline pre-LPS nebulisation (pre-LPS) and 6 hours post-LPS (N LPS- mice housed in normoxia post-LPS, H LPS- mice housed in hypoxia post-LPS). (Pre-LPS n = 6, N LPS n = 3, H LPS n = 3). (c) Blood was collected from mice treated with LPS and placed in normoxia (N LPS) or hypoxia 10% (H LPS) for 5 days and circulating neutrophils quantified by flow cytometry (Live Singles CD45+Ly6G+CD11b+). b, c Each data point represents an individual mouse. Data shown as mean±SEM. c two pooled independent experiments. Statistical testing performed using one-way ANOVA with Tukey’s multiple comparisons test.
