Extended Data Fig. 6: TRM differentiation programs are a source of intra-tissue heterogeneity.

a, UMAP dimensional reduction of scRNA-sequencing of TRM separated by tissue. Cells were colored by the expression of the indicated genes. Scales are consistent across tissues to allow for comparison within and among tissues. b-c, Expression of CD69, Ly6C, IL18R1 on P14 cells harvested 30-40 days after initial infection with LCMV. Representative flow cytometry plots (b) and quantification (c). d, Quantification of IL18R1 expression on P14 cells harvested from the indicated tissues 30-40 days after initial infection with LM-GP33. Quantification of flow cytometry data in c and d displays the mean ± SD for 6 (c) 10 (d) mice from 2 experimental replicates.