Extended Data Fig. 2: GPR65 I231L mice are more susceptible to bacterial infection-induced colitis.
(a,b) Colony forming units (CFU) in the stool (a) and body weight changes (b) of mice during C. rodentium infection. nWT = 6; nI231L = 7; nKO = 6 mice. (c) H&E-stained sections of distal colon from mice on day 3 after Citrobacter infection. Representative images from one of two independent experiments. Scale bar, 0.5 mm. (d) Statistical analysis of myeloid and CD4+ T cells in the colonic LP on day 12 after C. rodentium infection. nWT = 6; nI231L = 7; nKO = 6 mice. (e) Cytokine-producing CD4+ T cells in the mLN from WT (n = 6), I231L (n = 6) and KO (n = 6) mice on day 12 after infection. (f,g) Cytokine profiles of colonic tissue from C. rodentium-infected mice detected by qPCR (f) and multiplex bead-based cytokine assay (g). n = 4 mice per genotype (f); nWT = 6; nI231L = 7; nKO = 6 mice (g). (h-j) CFU, body weight change and colonic H&E histology of WT (n = 7), I231L (n = 7) or KO (n = 7) CD4+ T cell-transferred mice after Citrobacter infection. CFUs in colonic tissue and H&E staining were performed on day 12. (k,l) Immunophenotyping of CD4+ T cells and inflammatory innate immune cells in the mLN or colonic LP on day 12 after infection. n = 7 mice per genotype. (m) Cytokine profiles of colonic tissue on day 12 were detected by qPCR. n = 4 mice per genotype. Scale bar, 0.5 mm (c,j). Data represent at least two independent experiments. Data are mean values (a,b,d-i,k-m) + SEM (a,b,h,i). P values determined by unpaired two-tailed t-test; ns, not significant.
