Extended Data Fig. 4: TCF-1 and CTCF co-bound sites are evolutionarily conserved and insulated in non-T cells.
From: TCF-1 promotes chromatin interactions across topologically associating domains in T cell progenitors
a. Summary plot and heat map of sequence conservation using phastCons score at CTCF only, CTCF+TCF-1, TCF-1-only peaks and random regions as control. b. Local pileup plot of interactions using Hi-C measurements in progenitor and mature T cells in humans at homologs CTCF and TCF-1 peaks. Hi-C measurements were from human publicly available T-ALL, ETP T-ALL and ultra-deep Hi-C in CD8+ T cells from a healthy donor generated for this study. Human homologous regions for CTCF only, TCF-1 only as well as TCF-1+CTCF peaks in mice were found using liftOver. c. Local pileup plot of interactions at CTCF only, TCF-1+CTCF, TCF-1 only peaks in different mouse cell types, including mouse embryonic stem cell (mESC), fibroblast (NIH 3T3), cardiomyocyte, B cells, naïve CD4+ and DP T cells. d. Local pileup plot of interactions in different human cell types from 4D Nucleome repository at the liftOver sites of CTCF only, TCF-1 only as well as CTCF+TCF-1 peaks from mice.
