Extended Data Fig. 2: Loss of insulation and boundaries during T cell development.
From: TCF-1 promotes chromatin interactions across topologically associating domains in T cell progenitors
a. Summary plot and heat map of insulation score in six T cell developmental stages at CTCF only (red), CTCF+TCF-1 (yellow), TCF-1-only (blue) peaks and random regions as control (black), where lower value indicates higher insulation. Peaks defined in Fig. 1c. b. Violin plot and boxplot (center, median; box limits, upper (75th) and lower (25th) percentiles; whiskers, 1.5× interquartile range; points, outliers) showing differences in insulation score from CLP to DP at the four different sets of peaks. The comparison between different groups was done with one-sided Student’s t-test. The insulation score change was significantly (P < 2.2 × 10−16) different between different sets of peaks. c. Bar plot showing the total number of boundaries in six T cell developmental stages. d. Upset plot showing unique and shared boundaries among the six T cell developmental stages. The color shows different groups of boundaries that are lost or gained during T cell development. e. Local pileup in six T cell developmental stages at the nine groups of boundaries classified in (d). f. Quantification of interactions in six T cell developmental stages between upstream and downstream regions of the nine groups of boundaries.
