Extended Data Fig. 3: Colocalization of CTLA-4, ligand and markers of intracellular trafficking. | Nature Immunology

Extended Data Fig. 3: Colocalization of CTLA-4, ligand and markers of intracellular trafficking.

From: Differences in CD80 and CD86 transendocytosis reveal CD86 as a key target for CTLA-4 immune regulation

Extended Data Fig. 3

Confocal analysis of overnight transendocytosis between CHO CD80GFP (left-hand panels) or CD86GFP (right-hand panels) and HeLa cells expressing CTLA-4. Following transendocytosis cells were fixed and stained for CTLA-4 (red), ligand (green) and components of the endosomal/lysosomal pathway (Rab5, Rab7, Lamp3 and LRBA) as indicated (cyan). Arrowheads indicate location of triple colocalization of ligand, CTLA-4 and indicated cellular component. Arrows are illustrative and do not show all colocalization events, which were determined automatically using Cell Profiler software and are quantified in Figure 3b.

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