Extended Data Fig. 6: scRNA/TCR-seq reveals T cell clone behaviors in different organs.
From: Divergent clonal differentiation trajectories of T cell exhaustion
(a) Heat map of the fraction overlap between the TCR repertoires of the indicated gp33+ and gp33− CD8+ T cell subsets from different organs. (b) Stacked bar plot of the phenotypic composition of individual clones with divergent behavior across organs. (c) Schematic of the definition of an expanded, organ-shared T cell clone for clone behavior analysis. Only clones that had at least 5 T cells present in each organ were considered. Shared clone numbers across the organs are indicated (left). Table depicting the number of expanded clones that are shared across tissues and their clone behaviors (right). (d) Gene expression analysis of LCMV-gp transcript (left) and Il21 transcript (right) in indicated organs at D22 following Cl13 infection. Significant changes were determined by two tailed, unpaired t-test (n = 10 biologically independent animals). Box center line, mean; limits, upper and lower quartiles; whiskers, minimum and maximum values.
