Extended Data Fig. 2: DDX5 deficiency in keratinocytes drives skin inflammation.

a, Representative photos of ears from Ddx5^fl/fl(n = 5) and Ddx5^∆KC mice (n = 5)16 days post-MC903 administration. b, H&E analysis of skin sections from Ddx5^fl/fl and Ddx5^∆KC mice treated as in (a) and quantification of epidermal thickness of skin sections. Scale bar, 50μm. c, Representative photos of dorsal skin from Ddx5^fl/fl and Ddx5^∆KC mice 5 days post-IMQ administration. d, H&E analysis of skin sections from Ddx5^fl/fl and Ddx5^∆KC mice treated as in (c) and quantification of acanthosis of skin sections. Scale bar, 50μm. e, RT-qPCR of Il23, Il17a, Ccl20, Cxcl1, Cxcl2 and S100a7 in lesional skin treated as in (c). f, Flow cytometry of CD45⁺ cells, CD11b⁺Ly6G⁺ neutrophils, MHCII⁺ APCs and CD3⁺γδTCR⁺ cells in lesional dorsal skin from IMQ-treated Ddx5^fl/fl (n = 5) and Ddx5^∆KC mice (n = 5). Data represent two independent experiments. *P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001. P values were evaluated by unpaired, two-tailed Student’s t-test. Data are presented as mean ± s.e.m.

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