Extended Data Fig. 9: sIL-36R antagonizes IL-36R signaling to inhibit skin inflammation in keratinocyte, AD and psoriasis.
a, Flow cytometry analysis of the interaction between IL-36γ and IL-36R with or without sIL-36R overexpression in HeLa cells (n = 6). Data represent three independent experiments. b, RT-qPCR of CCL20, CXCL1, CXCL2, CXCL3, CCL3, CCL11 CCL17 and CCL27in NHEKs in response to IL-36γ after DDX5 was silenced and/or sIL-36R was overexpressed. Data represent three independent experiments. c, Representative photos of ears from MC903-treated (n = 5) or IMQ-treated (n = 5) wild-type mice injected with PBS in left ears or 1 μg per ear recombinant sIL-36R in right ears. d, RT-qPCR of Ccl20, Cxcl1, Il23 and Il17a in lesional skin from wild-type mice (n = 5) treated by IMQ as in (b). e, H&E staining of ear skin from wild-type mice treated by IMQ as in (b) and quantification of acanthosis of skin sections. f, Flow cytometry of CD45+ cells, CD11b+Ly6G+ neutrophils, CD11c+ APCs and CD3+γδTCR+ cells in ears from wild-type mice (n = 6) treated by IMQ as in (b). Data represent two independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001. P values were analyzed by two-way ANOVA (b) or unpaired, two-tailed Student’s t-test (a,d,e,f).
