Extended Data Fig. 4: Kinetics of CD4 T cell responses in the lung tissue following pulmonary M.tb infection. | Nature Immunology

Extended Data Fig. 4: Kinetics of CD4 T cell responses in the lung tissue following pulmonary M.tb infection.

From: Parenteral BCG vaccine induces lung-resident memory macrophages and trained immunity via the gut–lung axis

Extended Data Fig. 4: Kinetics of CD4 T cell responses in the lung tissue following pulmonary M.tb infection.

(a) Representative dotplots of lung mononuclear cells immunostained for surface markers CD4 and CD44 and gated to distinguish bona fide lung parenchymal T cells (LPT) from lung intravascular cells (LV) using CD45.2 antibody administered intravenously 3-5 min before sacrificing 5-wk PBS-treated or BCG-vaccinated mice. Data is from one experiment. (b) Mice were s.c. BCG-vaccinated or PBS-treated for 5-weeks. Airway AM harvested by bronchoalveolar lavage from PBS-treated (PBS-AM) and BCG-vaccinated (BCG-AM) were then adoptively transferred to naïve mice and challenged with M.tb. M.tb CFU in the lung at 3 days post-infection (n = 4 mice per group) were assessed. Data is from one experiment.

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