Fig. 1: Tissue expression of Nir2 and Nir3.
a, Normalized mRNA expression (nTPM) levels of PITPNM1 (Nir2) and PITPNM2 (Nir3) for 55 human tissue types, derived from the Human Protein Atlas database. b, Normalized expression values (generated from Immunological Genome Project datasets) of Pitpnm1 (Nir2) and Pitpnm2 (Nir3) in selected T cell subsets in thymus and spleen. c,d, Protein expression of Nir2 and Nir3 in thymocytes (Thy) and splenic T cells. c, Representative expression of Nir2 and Nir3 was assessed by immunoblots. Ectopic expression of Nir2-mCherry (Nir2-mCh) and Nir3-mCherry (Nir3-mCh) in HEK293 cells was included for normalization. d, The band intensities of Nir2 and Nir3 from four independent experiments similar to those shown in panel c were quantified and normalized to Nir2 expression in thymocytes. The relative abundance of Nir2 and Nir3 in each sample was first normalized to actin and was subsequently normalized to ectopic expression of Nir2-mCh or Nir3-mCh in HEK293 cells. Data are shown as the mean ± standard error of the mean (s.e.m.) and are collection of four independent experiments.
