Extended Data Fig. 6: Impaired development of Nir3–/– OT-II transgenic T cells. | Nature Immunology

Extended Data Fig. 6: Impaired development of Nir3–/– OT-II transgenic T cells.

From: The phosphatidylinositol-transfer protein Nir3 promotes PI(4,5)P2 replenishment in response to TCR signaling during T cell development and survival

Extended Data Fig. 6

Bone marrow cells from CD45.1/CD45.2 Nir3 sufficient (WT) OT-II mice and CD45.2 Nir3–/– OT-II mice were mixed at 1:1 ratio and adoptively transferred into CD45.1 hosts. 6 weeks after the transfer, WT OT-II and Nir3–/– OT-II thymocytes at each development stage and splenic T cells were identified by congenic CD45 markers. The percentages of WT OT-II cells and Nir3–/– OT-II cells in each stage were quantified. TN, T naive; TEM, T effector memory-like. N = 8 mice. Data are shown as the mean ± s.e.m. and are pooled from 2 independent experiments. P values were determined using a two-tailed Student’s t-test.

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