Fig. 5: Lymphopenia in Nir3–/– mice.
a–c, Total cell number in spleens (a), inguinal lymph nodes (b) and mesenteric lymph nodes (c) from WT and Nir3–/– female mice. d, Numbers of CD4 and CD8 cells in spleens from 8-week-old WT and Nir3–/– female mice. n = 5 mice. e–g, BM cells from 6- to 8-week-old CD45.1/CD45.2 Nir3 sufficient female mice and CD45.2 Nir3–/– female mice were mixed at a 1:1 ratio and adoptively transferred into 8-week-old CD45.1 female hosts for 6 weeks. e, The percentages of WT and Nir3–/– cells in each subset. DN, DN thymocytes; DP, DP thymocytes; CD4SP, CD4SP thymocytes; CD8SP, CD8SP thymocytes; CD4, splenic CD4 T cells; CD8, splenic CD8 T cells. n = 8 mice. f,g, WT and Nir3–/– splenic CD4 and CD8 T cells were subdivided into naive and memory-like subsets based on their expression of CD44 and CD62L. f,g, Representative flow plots (f) and quantification (g) of percentages of each subset. n = 4 mice. Data are shown as the mean ± s.e.m. and are representative of at least two independent experiments. P values were determined using an unpaired, two-tailed Student’s t-test.
