Fig. 7: Nir3 contributes to rapid PIP2 replenishment following receptor-induced hydrolysis in thymocytes. | Nature Immunology

Fig. 7: Nir3 contributes to rapid PIP2 replenishment following receptor-induced hydrolysis in thymocytes.

From: The phosphatidylinositol-transfer protein Nir3 promotes PI(4,5)P2 replenishment in response to TCR signaling during T cell development and survival

Fig. 7

a, Thymocytes and mature T cells from WT and Nir3–/– male mice were stimulated with anti-CD3 for the indicated times. PIP2 levels were normalized to unstimulated cells (t = 0). n = 4. b,c, OT-II CD4 T cells expressing Tubby-mScarlet were stimulated on coverslips coated with anti-CD3 antibody. Representative images (b) and quantification (c) of Tubby MFI on the spreading membrane are shown. d–i, OT-I CD8 T cells expressing Tubby-mScarlet were stimulated on coverslips coated with full (OVA), partial (T4) or weak (G4) agonist OVA peptide-MHC tetramers. Representative images (d,f,h) and quantification (e,g,i) of Tubby MFI on the spreading membrane were shown. Scale bar=10μm. N > 8 biological independent cells. All data are shown as the mean ± s.e.m. and are representative of at least two independent experiments. P values were determined using a paired, two-tailed Student’s t-test.

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