Fig. 8: Nir3 regulates TCR-induced [Ca2+]I in DP thymocytes. | Nature Immunology

Fig. 8: Nir3 regulates TCR-induced [Ca2+]I in DP thymocytes.

From: The phosphatidylinositol-transfer protein Nir3 promotes PI(4,5)P2 replenishment in response to TCR signaling during T cell development and survival

Fig. 8

WT or Nir3–/– DP thymocytes loaded with the calcium sensitive dye Indo-1 AM were incubated with indicated amount of anti-CD3 antibody at time 0 and stimulated with crosslinking antibodies, thapsigargin or ionomycin at the arrow. The [Ca2+]I elevation of each DP subset, as gated in Fig. 4a, was assessed based on the Indo-1 fluorescence emission ratio 405nm/485nm by flow cytometry. n > 5 cells. All data are shown as the mean ± s.e.m. and are representative of at least two independent experiments.

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