Extended Data Fig. 6: RNA-seq analysis reveals altered pathways in LPS or anti-IgM stimulated SCAP deficient B cells.
From: SREBP signaling is essential for effective B cell responses
Bead-purified splenic B cells from SCAP+/+ CD19Cre/+ mice and SCAPfl/fl CD19Cre/+ mice were stimulated with 10 μg/ml LPS or 20 μg/ml anti-IgM for 24 hours. RNA was isolated from the cells and analyzed by RNA sequencing. Pathways that were altered in SCAP deficient B cells post LPS (a) and anti-IgM (b) stimulation were ranked by FDR ( < 0.05). Shown are data from 2 independent experiments with cells pooled from 5 mice in each experiment.
