Extended Data Fig. 2: Differential integrin expression by in vitro and in vivo TH cells. | Nature Immunology

Extended Data Fig. 2: Differential integrin expression by in vitro and in vivo TH cells.

From: An αvβ3 integrin checkpoint is critical for efficient TH2 cell cytokine polarization and potentiation of antigen-specific immunity

Extended Data Fig. 2

Extended Data Fig. 2(a) Flow cytometric analysis of αv and β3 expression by TH2 cells in vitro. (b) Flow cytometric analysis of α4 (Itga4) and β1 (Itgb1) expression by TH cells in vitro. Data are representative of 2 independent experiments with 4 biologically independent samples in each experiment; unpaired two-sided t-test; ****P < 0.0001, ***P = 0.0004. (c) Gating strategy for TH cell subsets in the papain-challenged lung. (d) Flow cytometric gating strategy and quantification of lymphoid populations in naive mice. (e) Flow cytometric analysis of αv and β3 expression in control, αv- or β3- deficient naïve CD4+ T cells. (f) and (g) Quantification of lymphoid populations in naive mice. Data representative of 2 independent experiments with 3 biologically independent samples in each experiment; mean ± SD; unpaired two-sided t-test. (h) Schematic of the experimental induction of type 2 inflammation in the mouse lung with OVA/Alum. (i) Schematic of the experimental induction of type 2 inflammation in the mouse lung with papain. (j) Schematic of the experimental induction of type 1 inflammation in the mouse lung with LPS.

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